Cyanase-mediated utilization of cyanate in Pseudomonas fluorescens NCIB 11764.

Complete Genome Sequence of a Cyanotroph, Pseudomonas fluorescens NCIMB 11764, Employing Single-Molecule Real-Time Technology

We report here the application of single-molecule real-time sequencing for determining the entire genome structure of the cyanotroph Pseudomonas fluorescens NCIMB 11764.

REQUIREMENTS FOR CELL - FREE CYANIDE OXIDATION BY Pseudomonas fluorescens NCIMB 11764. Preeti B. Parab Thesis Prepared for the Degree of MASTER OF SCIENCE UNIVERSITY OF NORTH TEXAS

Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an enzyme that is not essential for cyanide assimilation.

Cyanase catalyzes the decomposition of cyanate into CO(2) and ammonium, with carbamate as an unstable intermediate. The cyanase of Pseudomonas pseudoalcaligenes CECT5344 was negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. Cyanase activity was not detected in cell extracts from cells grown with ammonium, even in the presence of cya...

The cyanate utilization capacity of marine unicellular Cyanobacteria

Cyanate, a by-product of urea decomposition, is a potential nitrogen (N) source in marine environments, but to date it has received scant attention. Cyanobacteria presumably acquire this compound via a substrate-specific ABC-type transporter (cynABD), and they convert it to ammonium and carbon dioxide by cyanase (cynS) activity. Participation of cyanate utilization genes in N-stress responses i...

Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp.

Cyanobacteria of the genera Synechococcus and Prochlorococcus are the most abundant photosynthetic organisms on earth, occupying a key position at the base of marine food webs. The cynS gene that encodes cyanase was identified among bacterial, fungal, and plant sequences in public databases, and the gene was particularly prevalent among cyanobacteria, including numerous Prochlorococcus and Syne...